Based on the information provided in the URL, we have the Human PRG4 (Proteoglycan 4) ELISA Kit from Assay Genie. This kit is specifically designed for the accurate detection of proteoglycan 4 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an excellent tool for various research applications.Proteoglycan 4 plays a crucial role in joint lubrication and cartilage function, making it an essential biomarker for studying conditions such as osteoarthritis and other joint-related disorders.
By accurately measuring proteoglycan 4 levels, researchers can gain valuable insights into disease progression and potential therapeutic interventions.Overall, the Human PRG4 ELISA Kit from Assay Genie is a valuable tool for researchers studying joint health and cartilage function, offering accurate and reliable results for furthering our understanding of these critical biological processes.
Product Name:
Human PRG4 (Proteoglycan 4) ELISA Kit
SKU:
HUES02673
Target:
Human PRG4 (Proteoglycan 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRG4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRG4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRG4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PRG4. You can calculate the concentration of Human PRG4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
91-102
90-102
Average (%)
94
97
95
1:4
Range (%)
86-101
86-99
85-97
Average (%)
93
92
91
1:8
Range (%)
88-100
81-92
84-98
Average (%)
95
88
91
1:16
Range (%)
90-106
84-94
87-100
Average (%)
97
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.43
10.47
30.78
4.53
10.91
27.96
Standard deviation
0.27
0.44
1.51
0.26
0.47
0.91
C V (%)
6.09
4.2
4.91
5.74
4.31
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PRG4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRG4 in samples. No significant cross-reactivity or interference between Human PRG4 and analogues was observed.
