The Human PRKA Alpha1 (Protein Kinase AMP-Activated Alpha 1) ELISA Kit is a reliable tool for the quantification of PRKA Alpha1 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.PRKA Alpha1, also known as AMP-activated protein kinase alpha 1, plays a crucial role in cellular energy homeostasis and metabolism regulation.
Dysregulation of PRKA Alpha1 has been linked to various diseases such as diabetes, obesity, and cancer, making it a valuable biomarker for understanding these conditions and identifying potential therapeutic targets.With its easy-to-use format and robust performance, the Human PRKA Alpha1 ELISA Kit from Assay Genie is a valuable tool for researchers studying the role of PRKA Alpha1 in health and disease.
Human PRKA alpha1 (Protein Kinase, AMP Activated Alpha 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRKAα1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRKAα1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRKAα1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PRKAα1. You can calculate the concentration of Human PRKAα1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
98-113
84-97
Average (%)
100
104
90
1:4
Range (%)
91-106
81-94
83-97
Average (%)
97
86
90
1:8
Range (%)
91-103
87-100
81-92
Average (%)
97
92
87
1:16
Range (%)
91-105
82-95
86-99
Average (%)
97
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
102
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
233.65
617.07
2015.98
230.93
567.56
2007.6
Standard deviation
13.18
28.88
86.49
12.1
23.44
85.93
C V (%)
5.64
4.68
4.29
5.24
4.13
4.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PRKAα1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRKAα1 in samples. No significant cross-reactivity or interference between Human PRKAα1 and analogues was observed.
