Human Pro-Collagen I alpha Polyclonal Antibody (CAB24863)
The Human Pro-Collagen I alpha Polyclonal Antibody (CAB24863) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is specifically designed to target the pro-collagen I alpha chain, a key component in collagen synthesis. With its high reactivity towards human samples, this antibody is ideal for use in Western blotting applications to detect and analyze pro-collagen I alpha in various cell types. Its specificity allows for accurate and reliable results, making it a trusted resource for studies in regenerative medicine, wound healing, and extracellular matrix remodeling.Pro-collagen I alpha is essential for maintaining the structural integrity of tissues and organs, making it a crucial focus in research on fibrosis, scarring, and connective tissue disorders.
This antibody is validated for use in WB, IHC-P, IF-P applications and has demonstrated reactivity against Human samples.
Product Name:
Human Pro-Collagen I alpha Polyclonal Antibody
SKU:
CAB24863
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Purification Method
Affinity purification
Gene ID
1277
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from U-138 MG cells using Human Pro-Collagen I alpha Rabbit pAb (CAB24863) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Human Pro-Collagen I alpha Rabbit pAb (CAB24863) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
