With the Human Pro-IL1B ELISA Kit from Assay Genie, researchers can accurately measure levels of pro-IL1B in human samples such as serum, plasma, and cell culture supernatants. This highly sensitive kit offers reliable and reproducible results, making it ideal for various research applications.Pro-IL1B is a key protein involved in the inflammatory response, playing a critical role in conditions such as autoimmune diseases, inflammatory disorders, and infections.
By studying pro-IL1B levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Human Pro-IL1B ELISA Kit provides researchers with a powerful tool for studying the role of pro-IL1B in health and disease, paving the way for new advancements in the field of inflammation research.
Product Name:
Human pro-IL-1b ELISA Kit
SKU:
HUES03554
Target:
Human pro-IL-1b ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human pro-IL1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human pro-IL1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human pro-IL1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human pro-IL1β. You can calculate the concentration of Human pro-IL1β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
88-100
100-112
Average (%)
97
94
106
1:4
Range (%)
90-102
86-101
84-98
Average (%)
96
92
90
1:8
Range (%)
90-104
80-92
88-103
Average (%)
95
86
94
1:16
Range (%)
93-108
87-102
84-94
Average (%)
99
93
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
93-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.89
224.82
984.59
100.16
208.88
930.96
Standard deviation
6.55
11.87
42.24
6.0
8.38
46.83
C V (%)
6.49
5.28
4.29
5.99
4.01
5.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human pro-IL1β concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human pro-IL1β in samples. No significant cross-reactivity or interference between Human pro-IL1β and analogues was observed.
