Human Pro-MMP-10 (Pro-Matrix Metalloproteinase 10) CLIA Kit
The Human Pro-MMP-10 (Pro-Matrix Metalloproteinase-10) CLIA Kit is a valuable tool for the quantitative measurement of Pro-MMP-10 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and reliable results for a variety of research applications.Pro-MMP-10 is a critical enzyme involved in the breakdown of extracellular matrix components, influencing processes such as tissue remodeling and inflammation.
Abnormal levels of Pro-MMP-10 have been associated with various diseases, including cancer, arthritis, and cardiovascular disorders, making it a key biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human Pro-MMP-10 CLIA Kit is a valuable tool for researchers looking to explore the role of Pro-MMP-10 in disease pathogenesis and progression, providing valuable insights into potential diagnostic and therapeutic strategies.
Product Name:
Human Pro-MMP-10 (Pro-Matrix Metalloproteinase 10) CLIA Kit
SKU:
HUES00890
Target:
Human Pro-MMP-10 (Pro-Matrix Metalloproteinase 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Pro-MMP-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pro-MMP-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pro-MMP-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Pro-MMP-10. You can calculate the concentration of Human Pro-MMP-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
98-111
87-101
Average (%)
94
104
93
1:4
Range (%)
95-113
93-105
96-111
Average (%)
103
99
103
1:8
Range (%)
95-111
92-106
95-109
Average (%)
102
100
102
1:16
Range (%)
89-104
95-107
97-112
Average (%)
96
101
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
104
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.95
150.27
361.72
50.79
152.82
371.2
Standard deviation
5.62
15.54
34.69
5.72
11.72
33.7
C V (%)
10.82
10.34
9.59
11.26
7.67
9.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Pro-MMP-10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pro-MMP-10 in samples. No significant cross-reactivity or interference between Human Pro-MMP-10 and analogues was observed.
