Description
Product Name: | Human Progesterone (P) ELISA Kit |
SKU: | HDES0117 |
Size: | 96T |
Target: | P |
Detection Method: | Competition ELISA |
Result Type: | Quantitative: Sensitivity 98.6%, Specificity 99.7% |
Sample Type: | Serum |
Assay Time: | 1 h 15 min |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the principle to detect the Tuberculosis (TB) IgG in human serum, www.vetassay-elab.com 2 Enzyme linked immunosorbent assay (ELISA) was used to detect the content of progesterone (P) in serum. Firstly, a sheep anti rabbit coated microplate was used to prepare a solid-phase secondary antibody. Then, the test serum, horseradish peroxidase labeled progesterone, and anti-progesterone antibody were added to form a coated secondary antibody anti progesterone antibody progesterone (HRP) complex. The binding amount of labeled progesterone was negatively correlated with the amount of progesterone in the serum. After color development, the absorbance value (OD value) was measured using an enzyme-linked immunosorbent assay (ELISA) reader, and the concentration absorbance curve was fitted using a computer or graph to calculate the progesterone content in the serum to be tested.