Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit
The Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit is specifically designed for the accurate detection and quantification of PDIA6 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.PDIA6 is a key protein involved in the regulation of protein folding and assembly, as well as in redox regulation within the cell. It plays a critical role in various cellular processes, including oxidative stress response and ER stress signaling.
Dysregulation of PDIA6 has been implicated in various diseases, such as cancer, neurodegenerative disorders, and autoimmune conditions, making it a valuable biomarker for studying these diseases and potentially developing targeted therapies.Overall, the Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit is an essential tool for researchers looking to explore the role of PDIA6 in health and disease, providing accurate and reliable measurement of PDIA6 levels in biological samples.
Product Name:
Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit
Product Code:
HUDL02249
Size:
96 Assays
Target:
PDIA6
Synonyms:
PDI-A6, TXNDC7, P5, ERp5, Thioredoxin Domain Containing 7(Protein Disulfide Isomerase), Protein Disulfide Isomerase-Associated 6, Endoplasmic reticulum protein 5
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
