Human Protein Kinase, cGMP Dependent Type I (PRKG1) ELISA Kit
The Human Protein Kinase cGMP-Dependent Type I (PRKG1) ELISA Kit is specially designed for the precise measurement of PRKG1 levels in human samples, including serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this ELISA kit provides accurate and reproducible results, making it an excellent tool for a variety of research applications.PRKG1 is a critical protein kinase that plays a key role in various cellular processes, including the regulation of smooth muscle relaxation, platelet function, and vasodilation.
Dysregulation of PRKG1 has been linked to conditions such as hypertension, heart failure, and erectile dysfunction, highlighting its importance as a potential therapeutic target and biomarker for these diseases.By utilizing the Human Protein Kinase cGMP-Dependent Type I (PRKG1) ELISA Kit, researchers can gain valuable insights into the role of PRKG1 in disease pathogenesis and potentially identify novel treatment strategies. Get accurate and reliable data with this cutting-edge ELISA kit from Assay Genie.
Product Name:
Human Protein Kinase, cGMP Dependent Type I (PRKG1) ELISA Kit
Product Code:
HUDL02403
Size:
96 Assays
Target:
PRKG1
Synonyms:
CGKI, PGK, PRKG1B, PRKGR1B, cGKI-BETA, cGKI-alpha, cGMP-dependent protein kinase 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
