Human Protein Tyrosine Phosphatase Like Protein A (PTPLA) ELISA Kit
The Human Protein Tyrosine Phosphatase-Like Protein A (PTPLA) ELISA Kit is a powerful tool for researchers looking to accurately detect and quantify levels of PTPLA in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it suitable for a wide range of research applications.PTPLA is an important protein that plays a role in various cellular functions including cell signaling, metabolism, and disease progression.
Dysregulation of PTPLA has been implicated in conditions such as cancer, diabetes, and neurodegenerative disorders, highlighting its significance as a potential biomarker for studying these diseases and developing targeted therapies.By utilizing the Human Protein Tyrosine Phosphatase-Like Protein A (PTPLA) ELISA Kit, researchers can gain valuable insights into the role of PTPLA in health and disease, paving the way for innovative discoveries and advancements in the field of biomedical research.
Product Name:
Human Protein Tyrosine Phosphatase Like Protein A (PTPLA) ELISA Kit
Product Code:
HUDL02457
Size:
96 Assays
Target:
PTPLA
Synonyms:
CAP, HACD1, Proline Instead Of Catalytic Arginine Member a, 3-hydroxyacyl-CoA dehydratase 1, Cementum Attachment Protein, Very-long-chain 3R-3-hydroxyacyl-CoA dehydratase 1
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
