The Human PROZ (Protein Z) CLIA Kit is a cutting-edge assay designed to quantitatively measure levels of Protein Z in human samples including serum, plasma, and cell culture supernatants. This kit offers unparalleled accuracy and precision, ensuring dependable and consistent results for a variety of research purposes.Protein Z is a key factor in the coagulation cascade, playing a critical role in maintaining hemostasis and preventing excessive blood clotting. Dysregulation of Protein Z levels has been associated with various thrombotic disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
With its superior performance and ease of use, the Human PROZ CLIA Kit is an essential tool for researchers and clinicians seeking to investigate the role of Protein Z in health and disease. Trust in this reliable assay to deliver precise measurements and valuable insights into the complex mechanisms of hemostasis and thrombosis.
Product Name:
Human PROZ (Protein Z) CLIA Kit
SKU:
HUES00678
Target:
Human PROZ (Protein Z)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PROZ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PROZ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PROZ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PROZ. You can calculate the concentration of Human PROZ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
86-98
93-108
Average (%)
93
93
100
1:4
Range (%)
97-108
99-111
88-99
Average (%)
103
104
93
1:8
Range (%)
86-99
103-116
93-107
Average (%)
93
110
98
1:16
Range (%)
86-98
91-107
87-99
Average (%)
91
99
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
106
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
83-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.21
219.74
673.01
92.02
199.36
736.64
Standard deviation
8.83
16.52
50.27
11.04
17.62
76.17
C V (%)
9.47
7.52
7.47
12.0
8.84
10.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PROZ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PROZ in samples. No significant cross-reactivity or interference between Human PROZ and analogues was observed.
