The Human PRSS2 (Protease Serine 2) CLIA Kit is a powerful tool for the accurate measurement of PRSS2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.PRSS2 is a key protease involved in the regulation of various physiological processes, including digestion, immune response, and tissue remodeling. Dysregulation of PRSS2 has been implicated in several diseases, such as inflammatory disorders, autoimmune conditions, and certain cancers, making it a valuable biomarker for studying disease mechanisms and developing targeted treatments.
With the Human PRSS2 CLIA Kit, researchers can gain valuable insights into the role of PRSS2 in health and disease, ultimately leading to advancements in diagnostics and therapeutic strategies. Order your kit today and unlock the potential of PRSS2 research.
Product Name:
Human PRSS2 (Protease, Serine, 2) CLIA Kit
SKU:
HUES00680
Target:
Human PRSS2 (Protease, Serine, 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRSS2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRSS2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRSS2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PRSS2. You can calculate the concentration of Human PRSS2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
97-112
85-97
Average (%)
93
103
91
1:4
Range (%)
86-96
97-112
98-114
Average (%)
91
104
105
1:8
Range (%)
87-100
84-97
86-98
Average (%)
92
91
93
1:16
Range (%)
98-112
93-105
87-99
Average (%)
104
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
97
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.35
3.58
0.48
1.24
3.43
Standard deviation
0.06
0.13
0.37
0.06
0.1
0.39
C V (%)
11.76
9.63
10.34
12.5
8.06
11.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PRSS2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRSS2 in samples. No significant cross-reactivity or interference between Human PRSS2 and analogues was observed.
