Human PTHrP (Parathyroid Hormone Related Protein) CLIA Kit
The Human PTHrP (Parathyroid Hormone-Related Protein) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PTHrP levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.PTHrP is a critical protein involved in calcium homeostasis and bone metabolism, playing a key role in conditions such as hypercalcemia, osteoporosis, and certain cancers.
Its detection is essential for understanding the pathophysiology of these diseases and developing effective treatments.With its high sensitivity and specificity, the Human PTHrP CLIA Kit is a valuable tool for researchers and clinicians studying PTHrP and its implications in various diseases. Get accurate and reliable results with this advanced assay kit from Assay Genie.
Product Name:
Human PTHrP (Parathyroid Hormone Related Protein) CLIA Kit
SKU:
HUES00841
Target:
Human PTHrP (Parathyroid Hormone Related Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PTHrP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTHrP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTHrP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PTHrP. You can calculate the concentration of Human PTHrP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-119
95-107
101-115
Average (%)
110
100
109
1:4
Range (%)
99-117
103-116
103-118
Average (%)
107
109
109
1:8
Range (%)
99-110
89-102
96-111
Average (%)
104
95
101
1:16
Range (%)
97-113
102-117
90-102
Average (%)
103
110
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.22
117.73
339.3
43.61
114.03
351.74
Standard deviation
5.08
9.5
32.61
4.23
8.11
26.06
C V (%)
12.63
8.07
9.61
9.7
7.11
7.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PTHrP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PTHrP in samples. No significant cross-reactivity or interference between Human PTHrP and analogues was observed.
