Human PTHrP (Parathyroid Hormone Related Protein) ELISA Kit
The Human PTHrP (Parathyroid Hormone-Related Protein) ELISA Kit is specifically designed for the accurate measurement of PTHrP levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.PTHrP is a vital protein involved in calcium homeostasis and bone metabolism, playing a key role in conditions such as hypercalcemia and bone-related disorders.
By accurately measuring PTHrP levels, researchers can better understand the mechanisms underlying these conditions and potentially develop targeted therapies.Overall, the Human PTHrP ELISA Kit is a valuable tool for researchers studying bone health, calcium regulation, and related disorders, providing precise quantification of PTHrP levels for in-depth analysis.
Product Name:
Human PTHrP (Parathyroid Hormone Related Protein) ELISA Kit
SKU:
HUES02506
Target:
Human PTHrP (Parathyroid Hormone Related Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTHrP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTHrP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTHrP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTHrP. You can calculate the concentration of Human PTHrP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
85-101
93-107
Average (%)
97
92
101
1:4
Range (%)
91-104
84-95
89-100
Average (%)
97
90
95
1:8
Range (%)
88-102
84-96
85-96
Average (%)
95
89
90
1:16
Range (%)
92-107
87-98
85-98
Average (%)
99
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-106
97
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.65
129.26
465.14
51.08
133.97
507.77
Standard deviation
3.54
5.48
22.75
2.57
6.74
25.69
C V (%)
6.72
4.24
4.89
5.03
5.03
5.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PTHrP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PTHrP in samples. No significant cross-reactivity or interference between Human PTHrP and analogues was observed.
