The Human PTN (Pleiotrophin) CLIA Kit is a highly sensitive assay designed for the precise measurement of pleiotrophin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled accuracy and consistency, ensuring dependable results for diverse research purposes.Pleiotrophin is a multifunctional growth factor known for its role in cell growth, differentiation, and tissue repair. Its dysregulation is associated with various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
By detecting and quantifying pleiotrophin levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.The Human PTN (Pleiotrophin) CLIA Kit is a valuable tool for investigating the role of pleiotrophin in health and disease, offering the precision and reliability necessary for cutting-edge research in the fields of biology, medicine, and drug development.
Product Name:
Human PTN (Pleiotrophin) CLIA Kit
SKU:
HUES00706
Target:
Human PTN (Pleiotrophin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PTN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PTN. You can calculate the concentration of Human PTN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
92-103
99-114
Average (%)
107
97
106
1:4
Range (%)
96-113
90-104
102-114
Average (%)
104
95
109
1:8
Range (%)
90-105
92-105
92-104
Average (%)
96
98
99
1:16
Range (%)
102-117
94-111
91-105
Average (%)
110
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
105
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
99-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.55
69.45
363.2
46.61
70.67
395.76
Standard deviation
4.66
6.2
29.78
5.5
6.83
45.39
C V (%)
10.95
8.93
8.2
11.8
9.66
11.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PTN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PTN in samples. No significant cross-reactivity or interference between Human PTN and analogues was observed.
