Human PUMA (P53 Upregulated Modulator of Apoptosis) CLIA Kit
The Human PUMA (p53 Upregulated Modulator of Apoptosis) CLIA Kit is a powerful tool for detecting and quantifying PUMA levels in human samples. With high sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.PUMA is a key regulator of apoptosis, playing a critical role in the programmed cell death process. Abnormalities in PUMA expression have been linked to a variety of diseases, including cancer and neurodegenerative disorders, making it an important biomarker for understanding these conditions and exploring potential therapeutic interventions.
With the Human PUMA CLIA Kit, researchers can easily measure PUMA levels in serum, plasma, and cell culture supernatants, enabling detailed investigation into the role of PUMA in disease progression and treatment outcomes. Don't miss out on this valuable tool for advancing your research efforts in the field of apoptosis and beyond.
Product Name:
Human PUMA (P53 Upregulated Modulator of Apoptosis) CLIA Kit
SKU:
HUES00816
Target:
Human PUMA (P53 Upregulated Modulator of Apoptosis)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PUMA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PUMA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PUMA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PUMA. You can calculate the concentration of Human PUMA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
84-98
102-119
Average (%)
101
91
108
1:4
Range (%)
94-110
102-118
99-117
Average (%)
102
109
107
1:8
Range (%)
86-100
90-106
89-103
Average (%)
92
96
96
1:16
Range (%)
96-108
104-119
91-101
Average (%)
102
110
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
95
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.88
189.88
808.92
105.48
199.47
840.14
Standard deviation
8.09
13.92
56.62
9.92
21.98
64.61
C V (%)
8.27
7.33
7.0
9.4
11.02
7.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PUMA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PUMA in samples. No significant cross-reactivity or interference between Human PUMA and analogues was observed.
