Human Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1) ELISA Kit
The Human Purinergic Receptor P2Y G-Protein Coupled 1 (P2RY1) ELISA Kit is specifically designed for the precise and accurate measurement of P2RY1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and specificity, ensuring robust and dependable results for a variety of research applications.P2RY1 is known to play a critical role in various cellular functions, particularly in the regulation of cell signaling and response to extracellular nucleotides. Dysregulation of P2RY1 has been linked to several pathophysiological conditions, including inflammation, cardiovascular diseases, and neurological disorders, making it a valuable target for therapeutic intervention and biomarker discovery.
With its advanced technology and user-friendly protocols, the Human Purinergic Receptor P2Y G-Protein Coupled 1 (P2RY1) ELISA Kit is an invaluable tool for researchers seeking to investigate the role of P2RY1 in health and disease, paving the way for potential diagnostic and therapeutic advancements in the field.
Product Name:
Human Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1) ELISA Kit
Product Code:
HUDL02161
Size:
96 Assays
Target:
P2RY1
Synonyms:
P2Y1, P2Y Purinoceptor 1, ATP receptor, Purinergic receptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
