The Human PYY (Peptide YY) CLIA Kit is a cutting-edge assay designed for the specific detection of PYY levels in human samples such as serum, plasma, and cell culture supernatants. This highly sensitive and reliable kit provides accurate and reproducible results, making it an indispensable tool for researchers in a variety of fields.PYY is a peptide hormone that plays a crucial role in regulating appetite and food intake. By measuring PYY levels, researchers can gain valuable insights into metabolic disorders, obesity, and other related conditions.
This kit is essential for studying the physiological functions of PYY and its potential implications in the development of new therapeutic approaches.With its exceptional performance and versatility, the Human PYY CLIA Kit is the ideal choice for researchers seeking to investigate the role of PYY in various physiological and pathological processes. Order yours today and take your research to the next level.
Product Name:
Human PYY (Peptide YY) CLIA Kit
SKU:
HUES00705
Target:
Human PYY (Peptide YY)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PYY. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PYY. You can calculate the concentration of Human PYY in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
98-115
90-103
Average (%)
104
106
95
1:4
Range (%)
87-97
90-104
95-111
Average (%)
92
96
102
1:8
Range (%)
91-104
103-119
94-107
Average (%)
99
109
100
1:16
Range (%)
89-105
96-112
91-108
Average (%)
96
103
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.94
58.18
178.19
24.38
63.39
167.28
Standard deviation
3.16
6.85
17.62
2.63
6.2
17.11
C V (%)
12.18
11.77
9.89
10.79
9.78
10.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PYY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed.
