The Human PYY (Peptide YY) ELISA Kit is specifically designed for the precise quantification of peptide YY levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes. Peptide YY is a key peptide hormone that plays a crucial role in regulating appetite and food intake. It is involved in the control of energy homestasis and has been implicated in various metabolic disorders such as obesity and diabetes.
Thus, the measurement of peptide YY levels is essential for understanding the mechanisms underlying these conditions and exploring potential therapeutic interventions. With its advanced technology and reliable performance, the Human PYY ELISA Kit from Assay Genie provides researchers with a valuable tool for studying the physiological and pathological functions of peptide YY in both basic and clinical research settings.
Product Name:
Human PYY (Peptide YY) ELISA Kit
SKU:
HUES02302
Target:
Human PYY (Peptide YY)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PYY. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PYY. You can calculate the concentration of Human PYY in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
95-107
92-104
Average (%)
104
101
98
1:4
Range (%)
92-105
82-93
85-97
Average (%)
97
87
91
1:8
Range (%)
91-106
87-101
85-98
Average (%)
97
92
92
1:16
Range (%)
94-109
83-92
87-100
Average (%)
100
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.33
303.3
971.46
107.16
318.05
955.53
Standard deviation
5.72
15.23
49.74
6.19
17.4
38.51
C V (%)
5.43
5.02
5.12
5.78
5.47
4.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PYY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed.
