The Human R-Spondin 3 (RSPO3) ELISA Kit is a powerful tool for the precise quantification of RSPO3 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit offers reliable and consistent results, making it perfect for various research applications.RSPO3 is a key regulator of the Wnt signaling pathway and has been implicated in various biological processes, including stem cell maintenance, tissue regeneration, and tumorigenesis.
Its dysregulation has been linked to certain diseases such as cancer, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.In summary, the Human R-Spondin 3 (RSPO3) ELISA Kit is a cutting-edge tool for researchers looking to explore the role of RSPO3 in health and disease, providing accurate and insightful data for further investigation.
Product Name:
Human R-Spondin 3 (RSPO3) ELISA Kit
Product Code:
HUDL02572
Size:
96 Assays
Target:
RSPO3
Synonyms:
CRISTIN1, PWTSR, THSD2, Thrombospondin,Type I,Domain Containing 2, Protein with TSP type-1 repeat, Roof plate-specific spondin-3
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
