Human RAB7A, Member RAS Oncogene Family (RAB7A) ELISA Kit
The Human Rab7A (Member Ras Oncogene Family Rab7A) ELISA Kit is specifically designed for the precise and accurate detection of Rab7A levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results that are suitable for a variety of research applications.Rab7A is a key protein belonging to the Ras oncogene family, playing a crucial role in regulating various cellular processes such as endocytic trafficking, autophagy, and lysosomal biogenesis.
Dysregulation of Rab7A has been implicated in the pathogenesis of numerous diseases including cancer, neurodegenerative disorders, and infectious diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human Rab7A ELISA Kit provides a reliable tool for researchers to accurately measure Rab7A levels in biological samples, furthering our understanding of its role in health and disease.
Product Name:
Human RAB7A, Member RAS Oncogene Family (RAB7A) ELISA Kit
Product Code:
HUDL02486
Size:
96 Assays
Target:
RAB7A
Synonyms:
RAB7, Ras-related protein Rab-7a
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
