Human RAB8B, Member RAS Oncogene Family (RAB8B) ELISA Kit
The Human Rab8B (Member of Ras Oncogene Family) ELISA Kit is specifically designed to accurately measure Rab8B levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.Rab8B is a protein belonging to the Ras oncogene family, and it plays a critical role in intracellular vesicle trafficking and cell signaling pathways.
Dysregulation of Rab8B has been implicated in various diseases, including cancer and neurological disorders, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.With its advanced technology and validated performance, the Human Rab8B ELISA Kit is an essential tool for researchers looking to explore the physiological functions and pathological roles of Rab8B in human health and disease.
Product Name:
Human RAB8B, Member RAS Oncogene Family (RAB8B) ELISA Kit
Product Code:
HUDL02487
Size:
96 Assays
Target:
RAB8B
Synonyms:
RAB-8b protein, ras-related protein Rab-8B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
