Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) CLIA Kit
The Human RAC1 (Ras-related C3 botulinum toxin substrate 1) CLIA Kit is a powerful tool for detecting levels of RAC1 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results for a variety of research applications.RAC1 is a key protein involved in cell signaling and cytoskeletal remodeling, playing a crucial role in processes such as cell migration, proliferation, and invasion.
Dysregulation of RAC1 has been implicated in various diseases including cancer, inflammatory disorders, and neurological conditions, highlighting its importance as a potential therapeutic target.By using the Human RAC1 CLIA Kit, researchers can gain valuable insights into the role of RAC1 in disease pathogenesis and progression, paving the way for the development of novel treatment strategies and personalized medicine approaches.
Product Name:
Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) CLIA Kit
SKU:
HUES00885
Target:
Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Rac1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Rac1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Rac1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Rac1. You can calculate the concentration of Human Rac1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-112
95-109
86-100
Average (%)
102
102
92
1:4
Range (%)
85-97
96-108
100-117
Average (%)
92
102
108
1:8
Range (%)
87-104
99-117
102-115
Average (%)
95
108
109
1:16
Range (%)
85-99
101-115
94-107
Average (%)
92
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.61
179.58
767.05
102.69
184.23
807.22
Standard deviation
7.87
14.51
70.57
8.86
16.19
58.77
C V (%)
8.32
8.08
9.2
8.63
8.79
7.28
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Rac1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human Rac1 in samples. No significant cross-reactivity or interference between Human Rac1 and analogues was observed.
