Human RANk (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit
The Human Rank (Receptor Activator of Nuclear Factor Kappa-B) CLIA Kit is a cutting-edge assay designed for the precise measurement of Rank levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable tool for a wide array of research applications.Rank, also known as Receptor Activator of Nuclear Factor Kappa-B, plays a crucial role in regulating bone metabolism and immune function.
Dysregulation of Rank has been linked to various diseases, including osteoporosis, autoimmune disorders, and certain cancers, highlighting its importance as a biomarker for disease research and therapeutic development.In summary, the Human Rank (Receptor Activator of Nuclear Factor Kappa-B) CLIA Kit offers researchers a reliable and efficient way to quantify Rank levels and gain deeper insights into its role in health and disease.
Product Name:
Human RANk (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit
SKU:
HUES01247
Target:
Human RANk (Receptor Activator of Nuclear Factor Kappa B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RANκ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANκ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANκ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RANκ. You can calculate the concentration of Human RANκ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
89-105
95-109
Average (%)
106
97
101
1:4
Range (%)
93-107
102-119
101-118
Average (%)
99
109
108
1:8
Range (%)
95-108
102-119
85-101
Average (%)
102
109
92
1:16
Range (%)
87-101
100-113
96-108
Average (%)
92
106
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
96-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
111.01
212.71
888.15
121.18
200.85
851.99
Standard deviation
9.56
18.25
78.87
10.68
14.24
96.53
C V (%)
8.61
8.58
8.88
8.81
7.09
11.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RANκ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RANκ in samples. No significant cross-reactivity or interference between Human RANκ and analogues was observed.
