Human RBP3 (Retinol Binding Protein 3, Interstitial) ELISA Kit
The Human RBP3 (Retinol Binding Protein 3) Interstitial ELISA Kit is a powerful tool for accurately measuring RBP3 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.RBP3 is a vital protein involved in the transport and metabolism of retinol (vitamin A) in the body.
It plays a crucial role in maintaining vision and overall eye health, making it a significant biomarker for studying retinol-related disorders and developing potential treatments.With its advanced technology and reliable performance, the Human RBP3 Interstitial ELISA Kit is an invaluable asset for researchers looking to explore the role of RBP3 in various health conditions and advance our understanding of retinol biology.
Product Name:
Human RBP3 (Retinol Binding Protein 3, Interstitial) ELISA Kit
SKU:
HUES02595
Target:
Human RBP3 (Retinol Binding Protein 3, Interstitial)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RBP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RBP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RBP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RBP3. You can calculate the concentration of Human RBP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
86-100
100-111
Average (%)
102
92
105
1:4
Range (%)
88-102
84-95
84-97
Average (%)
95
89
90
1:8
Range (%)
88-104
85-99
85-97
Average (%)
95
92
90
1:16
Range (%)
86-101
85-99
82-96
Average (%)
93
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.38
3.71
0.48
1.42
3.76
Standard deviation
0.03
0.06
0.15
0.03
0.07
0.15
C V (%)
5.66
4.35
4.04
6.25
4.93
3.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RBP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RBP3 in samples. No significant cross-reactivity or interference between Human RBP3 and analogues was observed.
