Human Receptor II For The Fc Region Of Immunoglobulin E (FceRII) ELISA Kit
The Human Receptor II for the Fc Region of Immunoglobulin E (FcεRII) ELISA Kit is a cutting-edge tool designed for the precise measurement of FcεRII levels in human biological samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable asset for a wide array of research applications.FcεRII is a key receptor involved in the immune response, specifically in the binding of IgE antibodies. Understanding the role of FcεRII is crucial in studying various allergic and immune-mediated diseases, including asthma, allergic rhinitis, and autoimmune disorders.
With the FcεRII ELISA Kit, researchers can dive deep into the mechanisms underlying these conditions and explore potential therapeutic interventions.Overall, the Human Receptor II for the Fc Region of Immunoglobulin E (FcεRII) ELISA Kit offers a reliable and efficient solution for investigating the function and regulation of FcεRII in human health and disease, paving the way for groundbreaking discoveries in the field of immunology.
Product Name:
Human Receptor II For The Fc Region Of Immunoglobulin E (FceRII) ELISA Kit
Product Code:
HUDL01035
Size:
96 Assays
Target:
FceRII
Synonyms:
CD23, FCER2, BLAST-2, CLEC4J, FCE2, IGEBF, C-type lectin domain family 4 member J, Immunoglobulin E-binding factor, Lymphocyte IgE receptor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
