SynonymsCD32,FCGR2A,FCG2,FCGR2A1,FCGR2,FCGR2B,FCGR2C,Fc Fragment Of IgG, Low Affinity IIa, Receptor,Low affinity immunoglobulin gamma Fc region receptor II-a
ReactivityHuman
Sample TypeSerum, Plasma And Other Biological Fluids
SKU:
HUDL01040
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P12318
Sensitivity:
15.25pg/mL
Range:
46.875-3000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD32,FCGR2A,FCG2,FCGR2A1,FCGR2,FCGR2B,FCGR2C,Fc Fragment Of IgG, Low Affinity IIa, Receptor,Low affinity immunoglobulin gamma Fc region receptor II-a
Human Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) ELISA Kit
The Human Receptor II for the FC Region of Immunoglobulin G (FCGRII) ELISA Kit is a cutting-edge tool designed for the precise detection and quantification of human FCGRII levels in a variety of biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a multitude of research applications.FCGRII is a crucial receptor involved in immune responses, particularly in the recognition and binding of antibody-antigen complexes.
Its role in mediating immune cell functions makes it a valuable target for studying autoimmune diseases, allergies, and inflammatory conditions. The FCGRII ELISA Kit provides researchers with a reliable means of investigating the role of FCGRII in various disease mechanisms and potential therapeutic interventions.
Product Name:
Human Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) ELISA Kit
Product Code:
HUDL01040
Size:
96 Assays
Target:
FcgRII
Synonyms:
CD32, FCGR2A, FCG2, FCGR2A1, FCGR2, FCGR2B, FCGR2C, Fc Fragment Of IgG, Low Affinity IIa, Receptor, Low affinity immunoglobulin gamma Fc region receptor II-a
Detection Method:
Sandwich
Range:
46.875-3000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
