Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha) CLIA Kit
The Human REG3 Alpha (Regenerating Islet-Derived Protein 3 Alpha) CLIA Kit is specifically designed for the precise measurement of REG3 Alpha levels in human samples, including serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit delivers accurate and reproducible results, making it perfect for various research applications.REG3 Alpha is a critical protein involved in tissue regeneration and immune response, playing a key role in conditions such as inflammatory bowel disease, gastrointestinal disorders, and autoimmune diseases.
By accurately measuring REG3 Alpha levels, researchers can gain valuable insights into these conditions and potentially develop targeted therapies for their treatment.Overall, the Human REG3 Alpha CLIA Kit from Assay Genie provides researchers with a powerful tool to study REG3 Alpha and its implications in various diseases, ultimately contributing to advancements in biomedical research and therapeutic interventions.
Product Name:
Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha) CLIA Kit
SKU:
HUES00737
Target:
Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human REG3α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG3α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG3α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human REG3α. You can calculate the concentration of Human REG3α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
93-108
103-117
Average (%)
93
99
110
1:4
Range (%)
93-108
100-117
102-120
Average (%)
100
107
109
1:8
Range (%)
86-99
97-111
95-109
Average (%)
92
102
102
1:16
Range (%)
94-110
93-108
85-97
Average (%)
101
98
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.01
3.62
13.56
2.16
3.94
14.19
Standard deviation
0.2
0.37
1.35
0.27
0.33
1.62
C V (%)
9.95
10.22
9.96
12.5
8.38
11.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human REG3α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REG3α in samples. No significant cross-reactivity or interference between Human REG3α and analogues was observed.
