Human REG4 (Regenerating Islet Derived Protein 4) CLIA Kit
The Human REG4 (Regenerating islet-derived protein 4) CLIA Kit is specifically designed to accurately detect levels of REG4 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.REG4 is a vital protein involved in tissue regeneration and repair processes, contributing to the maintenance of healthy cellular function. Dysregulation of REG4 expression has been linked to various diseases such as gastrointestinal cancers, inflammatory bowel disease, and pancreatitis.
Therefore, the detection of REG4 levels can serve as a valuable biomarker for studying and potentially treating these conditions.With its advanced technology and high-performance capabilities, the Human REG4 CLIA Kit provides researchers with a reliable tool for investigating the role of REG4 in disease pathogenesis and exploring potential therapeutic interventions. Order yours today from Assaygenie to advance your research in the field of molecular biology and medicine.
Product Name:
Human REG4 (Regenerating Islet Derived Protein 4) CLIA Kit
SKU:
HUES01175
Target:
Human REG4 (Regenerating Islet Derived Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human REG4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human REG4. You can calculate the concentration of Human REG4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
93-106
86-99
Average (%)
99
100
92
1:4
Range (%)
98-113
84-98
101-114
Average (%)
105
91
108
1:8
Range (%)
88-102
88-102
100-113
Average (%)
94
95
106
1:16
Range (%)
92-103
90-101
87-101
Average (%)
97
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.62
168.56
960.05
88.28
165.03
999.68
Standard deviation
9.35
12.84
71.14
7.83
15.96
100.67
C V (%)
9.68
7.62
7.41
8.87
9.67
10.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human REG4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REG4 in samples. No significant cross-reactivity or interference between Human REG4 and analogues was observed.
