The Human RELN (Reelin) ELISA Kit is specifically designed for the precise and reliable detection of reelin levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Reelin is a key protein involved in neurodevelopment, playing a crucial role in regulating neuronal migration and synaptic plasticity. Dysregulation of reelin has been linked to various neurological disorders, including Alzheimer's disease, schizophrenia, and autism spectrum disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and proven performance, the Human RELN ELISA Kit is an essential tool for researchers looking to investigate the role of reelin in neurological disorders and advance our understanding of brain development and function.
Product Name:
Human RELN (Reelin) ELISA kit
SKU:
HUES03210
Target:
Human RELN (Reelin) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RELN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RELN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RELN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RELN. You can calculate the concentration of Human RELN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
97-113
92-106
Average (%)
100
104
99
1:4
Range (%)
92-107
84-98
86-98
Average (%)
99
91
92
1:8
Range (%)
89-99
81-93
85-99
Average (%)
94
87
90
1:16
Range (%)
86-101
86-98
81-93
Average (%)
93
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.46
3.87
0.5
1.59
3.82
Standard deviation
0.03
0.08
0.18
0.03
0.08
0.14
C V (%)
6.12
5.48
4.65
6.0
5.03
3.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RELN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RELN in samples. No significant cross-reactivity or interference between Human RELN and analogues was observed.
