The Human RLN1 (Relaxin-1) ELISA Kit is specifically designed for the accurate measurement of Relaxin-1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Relaxin-1 is a hormone that plays a key role in reproductive processes, including pregnancy and parturition. Additionally, it has been implicated in various physiological processes such as cardiovascular function and immune response.
Its dysregulation has been linked to conditions like fibrosis, cardiovascular diseases, and inflammatory disorders.This ELISA kit provides researchers with a valuable tool for studying the role of Relaxin-1 in various biological processes and disease mechanisms, ultimately aiding in the development of potential therapeutic interventions.
Product Name:
Human RLN1 (Relaxin-1) ELISA Kit
SKU:
HUES02651
Target:
Human RLN1 (Relaxin-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RLN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RLN1. You can calculate the concentration of Human RLN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
97-114
95-112
Average (%)
96
105
102
1:4
Range (%)
89-104
85-100
89-103
Average (%)
95
91
94
1:8
Range (%)
90-104
85-99
83-93
Average (%)
95
91
88
1:16
Range (%)
93-107
86-100
81-94
Average (%)
99
93
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
91-101
96
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
276.66
569.76
1772.44
273.1
552.21
1854.12
Standard deviation
14.08
26.15
85.79
16.55
23.19
86.03
C V (%)
5.09
4.59
4.84
6.06
4.2
4.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RLN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RLN1 in samples. No significant cross-reactivity or interference between Human RLN1 and analogues was observed.
