The Human RLN2 (Relaxin 2) CLIA Kit is specifically designed for the precise measurement of relaxin 2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Relaxin 2 is a vital hormone involved in various physiological processes, such as pregnancy, cardiovascular function, and tissue remodeling.
Its dysregulation has been linked to conditions like heart failure, fibrosis, and reproductive disorders, making it a valuable biomarker for studying these diseases and developing potential treatments. By utilizing the Human RLN2 CLIA Kit, researchers can obtain reliable data on relaxin 2 levels in human samples, allowing for a better understanding of its role in health and disease.
Product Name:
Human RLN2 (Relaxin-2) CLIA Kit
SKU:
HUES00898
Target:
Human RLN2 (Relaxin-2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RLN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RLN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RLN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RLN2. You can calculate the concentration of Human RLN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
92-108
97-112
Average (%)
92
100
104
1:4
Range (%)
95-111
89-101
86-97
Average (%)
101
95
91
1:8
Range (%)
103-116
96-110
90-103
Average (%)
108
102
96
1:16
Range (%)
103-119
85-97
102-116
Average (%)
110
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
102
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.14
52.67
171.08
22.02
53.32
159.78
Standard deviation
2.35
4.69
18.77
2.42
4.4
10.43
C V (%)
11.12
8.9
10.97
10.99
8.25
6.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RLN2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed.
