Human RUNX2 (Runt-Related Transcription Factor 2) CLIA Kit
The Human RUNX2 (Runt-related transcription factor 2) CLIA Kit is specifically designed for the quantitative measurement of RUNX2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.RUNX2 is a key transcription factor involved in bone formation and development, playing a critical role in osteoblast differentiation and skeletal morphogenesis.
Dysregulation of RUNX2 has been implicated in various bone disorders, such as osteoporosis and osteosarcoma, as well as in other conditions like tooth development and cartilage formation.By accurately measuring RUNX2 levels, researchers can gain valuable insights into bone metabolism, skeletal growth, and the pathogenesis of bone-related diseases. The Human RUNX2 CLIA Kit provides a powerful tool for studying RUNX2 biology and identifying potential therapeutic targets for bone disorders.
Product Name:
Human RUNX2 (Runt-Related Transcription Factor 2) CLIA Kit
SKU:
HUES00550
Target:
Human RUNX2 (Runt-Related Transcription Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RUNX2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RUNX2. You can calculate the concentration of Human RUNX2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
98-113
98-113
Average (%)
106
106
104
1:4
Range (%)
89-105
101-115
99-116
Average (%)
96
109
106
1:8
Range (%)
87-98
100-114
90-103
Average (%)
93
106
95
1:16
Range (%)
95-110
93-107
91-107
Average (%)
101
99
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
99-115
105
Cell culture media (n=5)
90-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.29
435.85
1445.33
176.64
430.22
1567.02
Standard deviation
15.73
31.69
157.83
21.09
39.8
122.54
C V (%)
8.4
7.27
10.92
11.94
9.25
7.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RUNX2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RUNX2 in samples. No significant cross-reactivity or interference between Human RUNX2 and analogues was observed.
