Human S100 Calcium Binding Protein A10 (S100A10) ELISA Kit
The Human S100 Calcium Binding Protein A10 (S100A10) ELISA Kit is a valuable tool for the precise quantification of S100A10 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.S100A10 is a key protein involved in calcium signaling and cell migration, playing a critical role in various physiological processes.
Dysregulation of S100A10 has been linked to inflammation, cancer, and other diseases, making it a crucial biomarker for studying these conditions and developing potential therapeutic interventions.With its reliable performance and user-friendly design, the Human S100A10 ELISA Kit is an essential resource for researchers seeking to explore the functions and implications of S100A10 in human health and disease.
Product Name:
Human S100 Calcium Binding Protein A10 (S100A10) ELISA Kit
Product Code:
HUDL02580
Size:
96 Assays
Target:
S100A10
Synonyms:
ANX2L, ANX2LG, CAL1L, CLP11, GP11, P11, P10, Calpactin-1 light chain, Cellular ligand of annexin II, Annexin II Ligand,Calpactin I,Light Polypeptide
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
