Human S100A11 (S100 Calcium Binding Protein A11) CLIA Kit
The Human S100A11 (S100 Calcium Binding Protein A11) CLIA Kit is specifically designed for the quantitative detection of S100A11 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research purposes.S100A11 is a calcium-binding protein that plays a critical role in various cellular processes, including cell growth, differentiation, and inflammation. Elevated levels of S100A11 have been linked to conditions such as cancer, inflammatory diseases, and neurodegenerative disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With its precision and efficiency, the Human S100A11 CLIA Kit is an essential tool for researchers looking to explore the role of S100A11 in disease pathology and development. Experience the reliability and convenience of this kit for your research needs.For more information and to purchase the Human S100A11 CLIA Kit, please visit www.assaygenie.com/human-s100a11-s100-calcium-binding-protein-a11-clia-kit-hues00744/
Product Name:
Human S100A11 (S100 Calcium Binding Protein A11) CLIA Kit
SKU:
HUES00744
Target:
Human S100A11 (S100 Calcium Binding Protein A11)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100A11. You can calculate the concentration of Human S100A11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
85-97
85-99
Average (%)
105
92
91
1:4
Range (%)
95-108
94-107
93-103
Average (%)
101
101
98
1:8
Range (%)
93-109
86-102
94-108
Average (%)
99
93
102
1:16
Range (%)
89-103
101-117
86-100
Average (%)
96
107
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
91-106
99
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
218.13
537.35
1645.33
238.26
510.96
1712.52
Standard deviation
21.97
56.8
148.41
27.23
40.06
132.72
C V (%)
10.07
10.57
9.02
11.43
7.84
7.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100A11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A11 in samples. No significant cross-reactivity or interference between Human S100A11 and analogues was observed.
