Human S100A6 (S100 Calcium Binding Protein A6) CLIA Kit
The Human S100A6 (S100 Calcium Binding Protein A6) CLIA Kit is specifically designed for the quantitative detection of S100A6 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.S100A6 is a calcium-binding protein that plays a crucial role in various cellular processes, including cell cycle regulation, differentiation, and apoptosis.
Abnormal levels of S100A6 have been linked to numerous diseases, including cancer, inflammatory disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human S100A6 CLIA Kit is a reliable tool for researchers seeking to investigate the role of S100A6 in disease pathology and identify new diagnostic and treatment strategies.
Product Name:
Human S100A6 (S100 Calcium Binding Protein A6) CLIA Kit
SKU:
HUES00747
Target:
Human S100A6 (S100 Calcium Binding Protein A6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100A6. You can calculate the concentration of Human S100A6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
93-106
88-103
Average (%)
102
100
94
1:4
Range (%)
100-116
85-98
89-102
Average (%)
107
91
95
1:8
Range (%)
98-114
101-119
96-108
Average (%)
105
108
102
1:16
Range (%)
97-112
95-112
97-110
Average (%)
103
102
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
102
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.65
94.7
491.62
49.55
99.18
521.23
Standard deviation
4.97
9.87
41.44
5.14
7.61
46.65
C V (%)
9.62
10.42
8.43
10.37
7.67
8.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100A6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A6 in samples. No significant cross-reactivity or interference between Human S100A6 and analogues was observed.
