Human S100 Calcium Binding Protein A6 (S100A6) ELISA Kit
The Human S100 Calcium Binding Protein A6 (S100A6) ELISA Kit is a highly sensitive and specific assay designed for the precise detection of S100A6 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent tool for a variety of research applications.S100A6 is a vital protein involved in calcium signaling and regulating cell growth and differentiation. Abnormal levels of S100A6 have been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
As such, this ELISA kit is an essential tool for studying the role of S100A6 in these diseases and developing potential therapeutic interventions.Overall, the Human S100A6 ELISA Kit offers researchers a powerful tool for studying the function and significance of S100A6 in various disease states, providing valuable insights for future research and clinical applications.
Product Name:
Human S100 Calcium Binding Protein A6 (S100A6) ELISA Kit
Product Code:
HUDL02588
Size:
96 Assays
Target:
S100A6
Synonyms:
S100-A6, 2A9, 5B10, CABP, CACY, PRA, MLN 4, Calcyclin, Growth factor-inducible protein 2A9, Prolactin receptor-associated protein
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
