Human S100A7 (S100 Calcium Binding Protein A7) CLIA Kit
The Human S100A7 (S100 Calcium Binding Protein A7) CLIA Kit is specifically designed for the quantitative detection of S100A7 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, guaranteeing accurate and reproducible results for various research applications.S100A7, also known as psoriasin, is a calcium-binding protein that plays a crucial role in inflammatory responses and skin diseases.
It is involved in regulating cell growth, differentiation, and migration, making it a valuable biomarker for studying conditions like psoriasis, cancer, and inflammatory disorders. The Human S100A7 CLIA Kit allows for precise measurement of S100A7 levels, enabling researchers to further understand its function and potential therapeutic implications.
Product Name:
Human S100A7 (S100 Calcium Binding Protein A7) CLIA Kit
SKU:
HUES00748
Target:
Human S100A7 (S100 Calcium Binding Protein A7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100A7. You can calculate the concentration of Human S100A7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-105
103-117
102-117
Average (%)
96
109
109
1:4
Range (%)
101-118
87-99
96-109
Average (%)
108
93
102
1:8
Range (%)
87-99
92-106
98-114
Average (%)
92
97
105
1:16
Range (%)
97-110
95-111
95-110
Average (%)
104
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
101
EDTA plasma (n=5)
95-110
103
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
111.1
216.93
832.1
116.05
238.08
797.53
Standard deviation
14.33
22.67
90.28
15.06
25.93
89.08
C V (%)
12.9
10.45
10.85
12.98
10.89
11.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100A7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A7 in samples. No significant cross-reactivity or interference between Human S100A7 and analogues was observed.
