The Human Serum Amyloid A (SAA) CLIA Kit is a reliable and accurate tool for the measurement of SAA levels in human serum samples. This kit provides high sensitivity and specificity, allowing for precise and reproducible results in a variety of research applications. Serum Amyloid A is an important biomarker of inflammation and is associated with various inflammatory conditions such as rheumatoid arthritis, cardiovascular diseases, and infectious diseases.
Monitoring SAA levels can provide valuable insights into the progression of these diseases and the effectiveness of treatment interventions. The Human SAA CLIA Kit from AssayGenie is a valuable resource for researchers and healthcare professionals looking to study the role of SAA in disease pathology and develop new diagnostic and therapeutic strategies. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human SAA (Serum Amyloid A) CLIA Kit
SKU:
HUES01152
Target:
Human SAA (Serum Amyloid A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SAA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SAA. You can calculate the concentration of Human SAA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
100-117
100-112
Average (%)
107
109
106
1:4
Range (%)
100-115
101-118
98-111
Average (%)
106
109
105
1:8
Range (%)
101-116
99-112
87-99
Average (%)
107
105
93
1:16
Range (%)
98-115
97-113
93-110
Average (%)
105
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
3.19
7.62
1.15
3.02
7.34
Standard deviation
0.09
0.31
0.64
0.12
0.36
0.57
C V (%)
8.41
9.72
8.4
10.43
11.92
7.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SAA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SAA in samples. No significant cross-reactivity or interference between Human SAA and analogues was observed.
