The Human SAP (Serum Amyloid P-Component) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of Serum Amyloid P-Component levels in human samples, including serum and plasma. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for a variety of research studies.Serum Amyloid P-Component is a critical protein involved in the formation of amyloid fibrils, which are associated with various diseases such as Alzheimer's, rheumatoid arthritis, and systemic amyloidosis.
By accurately quantifying Serum Amyloid P-Component levels, researchers can gain insights into disease progression and explore potential therapeutic interventions.With its user-friendly format and reliable performance, the Human SAP CLIA Kit is an essential tool for researchers and clinicians seeking to unravel the complex mechanisms underlying amyloid-related disorders and develop targeted treatment strategies. Visit the link above to learn more about this innovative kit and its applications in biomedical research.
Product Name:
Human SAP (Serum Amyloid P Component) CLIA Kit
SKU:
HUES00734
Target:
Human SAP (Serum Amyloid P Component)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SAP. You can calculate the concentration of Human SAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
83-98
87-98
Average (%)
105
90
92
1:4
Range (%)
98-112
92-109
86-99
Average (%)
104
100
91
1:8
Range (%)
85-96
98-111
94-107
Average (%)
90
104
101
1:16
Range (%)
101-120
87-99
83-98
Average (%)
109
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
99-117
107
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.42
11.4
29.27
4.09
11.68
27.39
Standard deviation
0.49
1.08
2.83
0.43
1.2
2.87
C V (%)
11.09
9.47
9.67
10.51
10.27
10.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SAP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SAP in samples. No significant cross-reactivity or interference between Human SAP and analogues was observed.
