Human SBEM (Small Breast Epithelial Mucin) CLIA Kit
The Human SBEM (Small Breast Epithelial Mucin) CLIA Kit is a highly accurate and reliable tool for detecting SBEM levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with precise and reproducible results for a variety of research applications. SBEM is a critical protein associated with breast cancer and has been implicated in other diseases as well.
By measuring SBEM levels, researchers can gain valuable insights into disease progression, potential treatment options, and overall patient outcomes. This kit is essential for studying the role of SBEM in various health conditions and developing targeted therapies.
Product Name:
Human SBEM (Small Breast Epithelial Mucin) CLIA Kit
SKU:
HUES01130
Target:
Human SBEM (Small Breast Epithelial Mucin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SBEM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SBEM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SBEM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SBEM. You can calculate the concentration of Human SBEM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
99-114
88-102
Average (%)
103
106
94
1:4
Range (%)
98-110
95-110
87-98
Average (%)
105
101
92
1:8
Range (%)
91-104
90-105
87-103
Average (%)
97
97
94
1:16
Range (%)
94-112
98-114
85-99
Average (%)
102
104
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.27
549.6
1864.38
222.09
564.48
1935.3
Standard deviation
22.2
46.39
191.66
24.92
65.54
217.91
C V (%)
10.87
8.44
10.28
11.22
11.61
11.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SBEM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SBEM in samples. No significant cross-reactivity or interference between Human SBEM and analogues was observed.
