Human SCCA1 (Squamous Cell Carcinoma Antigen 1) CLIA Kit
The Human SCCA1 (Squamous Cell Carcinoma Antigen 1) CLIA Kit is a cutting-edge diagnostic tool specifically designed for the precise measurement of SCCA1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts unparalleled sensitivity and specificity, guaranteeing accurate and consistent results, thus making it an indispensable resource for a wide array of research endeavors.SCCA1 is a paramount biomarker associated with squamous cell carcinoma, a type of skin cancer with potential implications in other malignancies as well. Its detection and quantification are vital for early diagnosis, prognosis prediction, and treatment monitoring in cancer patients.
Additionally, SCCA1 plays a critical role in various physiological and pathological processes, rendering it an attractive target for therapeutic interventions in cancer and other diseases.With its state-of-the-art technology and proven proficiency, the Human SCCA1 CLIA Kit from Assay Genie is an essential asset for researchers, clinicians, and healthcare professionals striving to advance their understanding of SCCA1 biology and improve patient care outcomes.
Product Name:
Human SCCA1 (Squamous Cell Carcinoma Antigen 1) CLIA Kit
SKU:
HUES00962
Target:
Human SCCA1 (Squamous Cell Carcinoma Antigen 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SCCA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SCCA1. You can calculate the concentration of Human SCCA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-119
87-99
91-107
Average (%)
109
92
98
1:4
Range (%)
88-101
98-112
88-99
Average (%)
93
104
93
1:8
Range (%)
91-106
94-106
85-100
Average (%)
99
100
91
1:16
Range (%)
95-108
90-102
103-119
Average (%)
102
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.27
109.41
423.38
47.67
101.57
458.79
Standard deviation
5.65
10.89
41.11
4.6
7.94
43.26
C V (%)
11.95
9.95
9.71
9.65
7.82
9.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SCCA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCCA1 in samples. No significant cross-reactivity or interference between Human SCCA1 and analogues was observed.
