The Human SCF (Stem Cell Factor) CLIA Kit is specifically designed for the precise measurement of stem cell factor levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research purposes.Stem cell factor plays a vital role in regulating the survival, proliferation, and differentiation of stem cells, making it a key player in tissue regeneration and repair processes.
Dysregulation of stem cell factor levels has been linked to various diseases and disorders, emphasizing its importance as a biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human SCF (Stem Cell Factor) CLIA Kit provides researchers with a valuable tool for investigating the role of stem cell factor in normal physiological processes and pathological conditions, paving the way for new discoveries and advancements in the field of stem cell biology.
Product Name:
Human SCF (Stem Cell Factor) CLIA Kit
SKU:
HUES00751
Target:
Human SCF (Stem Cell Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SCF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SCF. You can calculate the concentration of Human SCF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
83-97
101-116
Average (%)
100
90
109
1:4
Range (%)
102-119
91-105
96-109
Average (%)
109
98
102
1:8
Range (%)
97-109
102-117
90-105
Average (%)
102
109
96
1:16
Range (%)
100-114
101-118
101-116
Average (%)
107
108
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
93-105
100
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.31
133.07
424.59
52.42
140.94
385.49
Standard deviation
5.26
10.22
42.59
5.23
12.15
25.44
C V (%)
10.06
7.68
10.03
9.98
8.62
6.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SCF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCF in samples. No significant cross-reactivity or interference between Human SCF and analogues was observed.
