The Human SCF (Stem Cell Factor) ELISA Kit is specifically designed for the precise measurement of stem cell factor levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees reliable and consistent results, making it an excellent choice for various research purposes.Stem cell factor, also known as c-kit ligand, is a critical cytokine involved in the regulation of stem cell proliferation, differentiation, and survival. Its role in hematopoiesis, melanogenesis, and gametogenesis makes it a valuable target for studying various diseases and developing innovative therapies.
By utilizing the Human SCF ELISA Kit, researchers can effectively measure and monitor stem cell factor levels, advancing their understanding of stem cell biology and potentially identifying new treatment strategies for conditions such as cancer, blood disorders, and immune system abnormalities. Trust in the accuracy and precision of this ELISA kit for your research needs.
Product Name:
Human SCF (Stem Cell Factor) ELISA Kit
SKU:
HUES02355
Target:
Human SCF (Stem Cell Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SCF. You can calculate the concentration of Human SCF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
99-110
88-99
Average (%)
104
104
94
1:4
Range (%)
88-104
86-99
82-93
Average (%)
95
93
88
1:8
Range (%)
92-104
85-97
86-100
Average (%)
97
92
91
1:16
Range (%)
89-103
80-92
86-99
Average (%)
96
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
96-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.58
274.64
883.91
116.1
293.04
841.62
Standard deviation
5.82
11.81
44.64
7.83
13.48
25.33
C V (%)
5.31
4.3
5.05
6.74
4.6
3.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SCF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCF in samples. No significant cross-reactivity or interference between Human SCF and analogues was observed.
