The Human SCG2 (Secretogranin II) CLIA Kit is specifically designed for the quantitative detection of secretogranin II levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for various research applications.Secretogranin II is a key protein involved in the regulation of secretory granules and neurotransmitter release, playing a vital role in neuroendocrine signaling and secretory pathways. Abnormal levels of secretogranin II have been associated with various diseases, including neurological disorders and endocrine dysfunctions, making it a valuable biomarker for understanding these conditions and developing potential therapeutic strategies.
Overall, the Human SCG2 CLIA Kit provides a precise and efficient tool for researchers to study secretogranin II and its implications in health and disease. Visit assaygenie.com/human-scg2-secretogranin-ii-clia-kit-hues00516/ for more information on this innovative kit.
Product Name:
Human SCG2 (Secretogranin II) CLIA Kit
SKU:
HUES00516
Target:
Human SCG2 (Secretogranin II)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SCG2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCG2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCG2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SCG2. You can calculate the concentration of Human SCG2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
91-104
98-112
Average (%)
94
98
106
1:4
Range (%)
92-107
86-102
90-108
Average (%)
98
93
98
1:8
Range (%)
88-99
101-119
87-103
Average (%)
94
109
94
1:16
Range (%)
104-121
87-100
92-103
Average (%)
110
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.23
8.01
1.09
2.43
7.57
Standard deviation
0.09
0.16
0.6
0.11
0.26
0.55
C V (%)
8.91
7.17
7.49
10.09
10.7
7.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SCG2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCG2 in samples. No significant cross-reactivity or interference between Human SCG2 and analogues was observed.
