The Human SDC1 (Syndecan-1) CLIA Kit is specifically designed to accurately measure levels of Syndecan-1 in human serum, plasma, and cell culture supernatants. Syndecan-1 is a key protein involved in cell adhesion and signaling, playing a critical role in various physiological and pathological processes.This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for your research needs. Syndecan-1 has been implicated in numerous diseases, including cancer, inflammation, and fibrosis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its user-friendly protocol and superior performance, the Human SDC1 CLIA Kit is an essential tool for researchers looking to advance their understanding of Syndecan-1 biology and its implications in disease pathology. Order yours today and take your research to the next level.
Product Name:
Human SDC1 (Syndecan 1) CLIA Kit
SKU:
HUES00750
Target:
Human SDC1 (Syndecan 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SDC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SDC1. You can calculate the concentration of Human SDC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
94-107
87-103
Average (%)
109
100
95
1:4
Range (%)
96-109
95-110
85-98
Average (%)
104
102
92
1:8
Range (%)
85-100
99-113
94-110
Average (%)
92
106
101
1:16
Range (%)
89-104
93-105
101-118
Average (%)
96
98
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
98-110
104
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.9
175.19
799.55
99.93
183.61
864.05
Standard deviation
11.88
16.21
70.6
8.02
15.26
70.07
C V (%)
11.55
9.25
8.83
8.03
8.31
8.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SDC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SDC1 in samples. No significant cross-reactivity or interference between Human SDC1 and analogues was observed.
