The Human Secretagogin (SCGN) ELISA Kit is specially designed for the precise measurement of secretagogin levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for various research applications.Secretagogin is a key protein involved in calcium signaling and regulation, playing a vital role in various physiological processes including insulin secretion, neuronal development, and cell survival.
Dysregulation of secretagogin has been linked to various diseases such as diabetes, neurodegenerative disorders, and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human Secretagogin ELISA Kit offers researchers a reliable tool for investigating the role of secretagogin in health and disease, paving the way for advancements in diagnosis, treatment, and understanding of various medical conditions.
Product Name:
Human Secretagogin (SCGN) ELISA Kit
Product Code:
HUDL02615
Size:
96 Assays
Target:
SCGN
Synonyms:
SECRET, SEGN, CALBL, Calbindin-Like, Secretagogin,EF-Hand Calcium Binding Protein
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
