The Human SELE (E-Selectin) CLIA Kit is a powerful tool for the quantitative measurement of E-Selectin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.E-Selectin is a cell adhesion molecule that plays a crucial role in inflammation and leukocyte recruitment to sites of tissue damage or infection.
Elevated levels of E-Selectin have been associated with various inflammatory conditions and cardiovascular diseases, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With its easy-to-use format and robust performance, the Human SELE CLIA Kit from Assay Genie is an essential tool for researchers seeking to unravel the complexities of E-Selectin biology and its impact on human health.
Product Name:
Human SELE (E-Selectin) CLIA Kit
SKU:
HUES00529
Target:
Human SELE (E-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SELE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SELE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SELE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SELE. You can calculate the concentration of Human SELE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
91-106
102-117
Average (%)
107
99
109
1:4
Range (%)
99-113
99-115
85-100
Average (%)
107
106
92
1:8
Range (%)
98-115
98-113
92-106
Average (%)
105
106
98
1:16
Range (%)
86-99
85-97
86-99
Average (%)
92
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.02
122.6
405.49
45.89
131.36
437.19
Standard deviation
4.37
13.15
36.9
4.51
10.61
31.57
C V (%)
8.91
10.73
9.1
9.83
8.08
7.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SELE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SELE in samples. No significant cross-reactivity or interference between Human SELE and analogues was observed.
