The Human sELl (L-Selectin) CLIA Kit is a cutting-edge assay designed to accurately measure levels of sELl (L-Selectin) in human biological samples such as serum, plasma, and cell culture supernatants. This innovative kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.sELl (L-Selectin) is a critical cell adhesion molecule that plays a key role in inflammatory responses and immune cell trafficking. Abnormal levels of sELl (L-Selectin) have been associated with various inflammatory and autoimmune conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With its advanced technology and reliability, the Human sELl (L-Selectin) CLIA Kit is a must-have tool for researchers investigating the role of sELl (L-Selectin) in health and disease. Order yours today from Assay Genie to accelerate your research efforts and make groundbreaking discoveries in the field of immunology.
Product Name:
Human SELL (L-Selectin) CLIA Kit
SKU:
HUES00536
Target:
Human SELL (L-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SELL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SELL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SELL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SELL. You can calculate the concentration of Human SELL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
96-112
91-103
Average (%)
106
104
98
1:4
Range (%)
101-113
101-118
90-105
Average (%)
107
109
97
1:8
Range (%)
90-106
97-114
95-112
Average (%)
97
105
102
1:16
Range (%)
99-113
89-104
102-117
Average (%)
105
95
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.75
419.1
1602.39
234.88
439.77
1559.53
Standard deviation
24.5
45.72
147.74
28.37
47.98
159.38
C V (%)
11.15
10.91
9.22
12.08
10.91
10.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SELL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SELL in samples. No significant cross-reactivity or interference between Human SELL and analogues was observed.
