The Human SELP (P-Selectin) CLIA Kit is a cutting-edge assay designed to accurately measure levels of P-Selectin in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.P-Selectin is a vital cell adhesion molecule that plays a key role in inflammatory responses and the recruitment of immune cells to sites of injury or infection. Elevated levels of P-Selectin have been implicated in various diseases, including cardiovascular disorders, inflammatory conditions, and thrombotic events, making it a valuable biomarker for studying these pathologies and developing targeted treatments.
With its user-friendly format and reliable performance, the Human SELP CLIA Kit is an indispensable tool for researchers looking to deepen their understanding of P-Selectin biology and its implications in human health and disease. Order yours today and unlock new insights into this critical molecular pathway.
Product Name:
Human SELP (P-Selectin) CLIA Kit
SKU:
HUES00547
Target:
Human SELP (P-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SELP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SELP. You can calculate the concentration of Human SELP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
86-101
93-107
Average (%)
100
93
99
1:4
Range (%)
92-106
94-107
98-115
Average (%)
99
100
106
1:8
Range (%)
103-117
90-103
90-106
Average (%)
110
97
97
1:16
Range (%)
87-100
97-112
102-118
Average (%)
94
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
90-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.81
237.29
912.71
110.52
259.31
912.48
Standard deviation
12.74
27.22
84.24
13.15
27.31
89.42
C V (%)
12.16
11.47
9.23
11.9
10.53
9.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SELP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SELP in samples. No significant cross-reactivity or interference between Human SELP and analogues was observed.
