The Human SEMA7A (Semaphorin 7A) CLIA Kit is a highly efficient tool for the precise measurement of SEMA7A levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, thus making it a valuable asset for various research applications.SEMA7A, also known as Semaphorin 7A, is a critical protein involved in cell signaling and regulation, particularly in processes such as immune response, neuronal development, and inflammation.
Dysregulation of SEMA7A has been associated with various diseases including cancer, autoimmune disorders, and neurological conditions, highlighting its importance as a biomarker for disease progression and therapeutic interventions.Overall, the Human SEMA7A CLIA Kit provides researchers with a reliable and efficient means of studying SEMA7A levels, thereby advancing our understanding of its role in health and disease.
Product Name:
Human SEMA7A (Semaphorin 7A) CLIA Kit
SKU:
HUES00632
Target:
Human SEMA7A (Semaphorin 7A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA7A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA7A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA7A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SEMA7A. You can calculate the concentration of Human SEMA7A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
88-100
86-99
Average (%)
99
94
92
1:4
Range (%)
89-100
98-109
89-100
Average (%)
95
104
94
1:8
Range (%)
103-119
102-115
100-116
Average (%)
110
108
107
1:16
Range (%)
100-119
92-104
92-108
Average (%)
108
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
103
EDTA plasma (n=5)
100-114
107
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.72
188.85
770.92
98.46
176.95
794.75
Standard deviation
8.14
20.45
52.5
11.03
14.24
78.28
C V (%)
8.16
10.83
6.81
11.2
8.05
9.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SEMA7A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SEMA7A in samples. No significant cross-reactivity or interference between Human SEMA7A and analogues was observed.
